---
title: "What is Recombinant DNA Technology?"  
description: "What is Recombinant DNA Technology?"  
author: "Tarun Gautam"  
published: 2017-11-16  
canonical: https://answers.mindstick.com/qa/30794/what-is-recombinant-dna-technology  
category: "technology"  
reading_time: 2 minutes  

---

# What is Recombinant DNA Technology?

What is Recombinant DNA Technology?

## Answers

### Answer by UTKARSHA SINGHROUL

**‘Recombinant DNA’ or rDNA [Technology](https://yourviews.mindstick.com/view/332/artificial-intelligence-bane-or-boon) is the process of joining DNA molecules taken from two different sources and inserting that combined [DNA](https://answers.mindstick.com/qa/49700/who-invented-the-dna-structure) into a host body**, such that to produce the product usable for humans. Or, we can say, Recombinant DNA is formed in a laboratory by following the methods to bring out a combination of two different genes.

![What is Recombinant DNA Technology?](https://answers.mindstick.com/questionanswer/dc98370c-363e-4163-a162-5ee2b2ce694e/images/b879f7bb-4f19-4476-896e-48593647fd5a.jpeg)\
The **changes or reformation in DNA is only possible because the DNA molecules share the same molecule [structure](https://answers.mindstick.com/qa/39696/the-parliament-of-india-has-passed-a-law-extending-the-deadline-by-three-years-for-protection-of-illegal-structures-in-which-city) in all the organisms**. Whereas, the change only occurs in the sequence of nucleotide within the overall [identical](https://answers.mindstick.com/qa/31674/why-do-identical-twins-have-different-fingerprints) structure. Recombinant DNA molecules are sometimes also referred to as “Chimeric DNA”. The **basic concept of recombining the molecular structure of DNA was given by “Warner Arber”**. Due to which the doctors learned about ‘cut’ and ‘paste’ of DNA molecules. Later in **1972, the [experiment](https://answers.mindstick.com/qa/42848/why-did-the-puritan-experiment-in-the-new-world-last-only-a-few-short-generations) has been advanced by the “Stanley Cohen” and “Herbert Boyer” to provide the Recombinant DNA Technology.** The Recombinant DNA technology is mainly based on **three tools i.e.; Enzymes, Vectors, and Host Organism**. These **‘Enzymes’** helps to cut, synthesize, and bind the DNA structure. The **‘Vectors’** carry the required gene and are an important part of recombinant DNA. The **‘Host Organism’** is the cell in which the recombinant DNA will be introduced. The **host organism can be [Bacteria](https://answers.mindstick.com/qa/31570/india-has-been-declared-free-from-trachoma-which-is-a-contagious-bacterial-infection-of-which-part-of-human-body), Fungi, or an Animal Cell.** To carry out the introduction of newly formed recombinant DNA Vectors into the host organism, **different technologies can be used and it includes, Biolistics, Microinjection, Gene Gun, Alternate Cooling and Heating, and Calcium Phosphate ions**. The introduction of recombinant DNA into the host organism, this whole process is named as ‘transformation’. \
Generally, recombinant DNA technology follows these five steps: **1.** By restricting the desired sites cut the DNA, **2.** Escalate or Amplify the copies of the gene by using PCR, **3.** Now, introduce the genes into the Vectors, **4.** Then, transfer the vector genes into the host organism, and **5.** Now, you can get the resultant product of recombinant genes.


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Original Source: https://answers.mindstick.com/qa/30794/what-is-recombinant-dna-technology

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