The Natchez Trace also called the Old Natchez Trace, is a 440-mile (710-kilometer) ancient woodland route in the U.S. that connects the Cumberland, Tennessee, and Mississippi rivers.

Native Americans constructed and utilized the path for centuries before it was used by early European and American adventurers, merchants, and immigrants in the late eighteenth and early ninth centuries. Following the Trace, European Americans established inns, often known as 'stands,' to provide food and shelter to visitors. As traffic on the Mississippi and other rivers changed to steamships, many of these stands disappeared.

The Natchez Trace Parkway, which traces the general course of the Trace, and the connected Natchez Trace Trail memorialize the path today. Some sections of the original path are still available, and some are on the National Register of Historic Places.